Microscope, Microscopy
BESTSCOPE: Confocal Microscope
- Confocal Microscope works by allowing only light from a single focal plane to reach the detector through a pinhole which is positioned precisely to be in focus with the sample’s focal plane. The light source excites the sample at a specific point and the emitted light travels back through the same optical path to a pinhole placed in front of a detector that generates an electrical signal proportional to the amount of light detected. The point of excitation scans across the sample capturing electrical signals to be compiled into an image.
- Refer to Datasheet of various models below.
- BCF296
- High-Performance Confocal Microscopy Platform: When paired with BS-2096 inverted microscope, it offers a powerful and flexible imaging solution for microscopic imaging within the BCF296 system. The large field of view of 25mm reduces the number of images required for stitching large images and decreases image acquisition time with uniform brightness, providing ideal observation for large sample. Integrating various microscopical techniques such as brightfield, fluorescence, differential interference contrast, and phase contrast, single-layer or dual-layer optical path configurations can be selected to achieve optimal imaging results. The Adaptive Focus Shift System (AFS) ensures precise focal plane positioning during continuous observations, enabling stable, continuous, and clear recordings of cellular dynamic behaviours.
- High-performance objectives for confocal imaging: The NIS series objectives with multi-layer coating technology boast high numerical apertures, long working distances, and exceptional chromatic aberration correction capabilities
- High-efficiency scanning heads and detectors: Internally integrated high-precision scanning galvanometer system within its scanning head, coupled with a continuously variable electric pinhole that enables low noise, high contrast, and premium quality confocal imaging. With a scanning size of 8192 x 8192 pixels, it transcends the optical diffraction limit even when using low magnification objectives.
- Fast Scanning Speed: High-speed imaging up to 60 frames per second (fps) at 8 x 256 pixels, reducing exposure time for samples under high light intensities and significantly decreasing phototoxicity. This rapid imaging speed facilitates high-frequency data acquisition, enabling the capture of dynamic events and prolonged changes in samples, accurately and in real-time, fulfilling the complex and stringent imaging requirements of the life sciences domain.
- Real-Time 4-Channel Synchronized Imaging: Incorporates cutting-edge 4-channel fluorescence merging technology; conduct in real-time, synchronous, multi-channel precise fusion observations and captures. Allow simultaneous detection and analysis of 4 distinct fluorescent labels within the same field of view, seamlessly switching and integrating multiple signals. Coupled with precise spectral unmixing imaging, this technology vividly and three-dimensionally reveals intricate, multi-layered information within samples.
- Integrated Software Platform for Advanced Analysis and Visualization: Adaptation software achieves a high level of integration and control over the hardware devices of the confocal system and the core functions of the microscope. It seamlessly combines these controls with confocal imaging analysis, creating a high-performance, user-friendly, all-in-one experimental solution.
- Multi-Dimensional Imaging and Display: Capable of memorizing custom observation modes and supports the combined use of X, Y, Z, λ, and T scanning functions. Equipped with a variety of flexible shooting modes, including multi-channel fluorescence imaging, time-lapse scanning, multi-position acquisition, Z-axis stacking, and panoramic stitching that can be combined for adaption to a wide range of complex and diverse experimental application scenarios.
- BCF297
- Motorized Control System: Adjust Z-axis height according to the real-time image using AutoFocus (AF) one-key function. Integrated control buttons on both sides of the frame for quick switch or rotate the condenser, brightness, objective lens, attenuator disc and fluorescence disc.
- Multiband LED Fluorescence Illumination System: Equipped with multiple excitation channels for quick switching to different experimental needs. Advanced light intensity control technology ensures optimal illumination. Supports live-cell observation expansion.
- Innovative Confocal Pinhole Unit: Based on the principle of light reversibility; excitation light of the lamp and the emission light of the sample pass through the same pinhole and keeping 100% conjugate relationship. Ensures the acquisition efficiency of fluorescence signal and improves the filtering of non-focal plane signal, for the higher detection sensitivity and better image resolution.
- Controller Detection Unit: High Sensitive GaAsP technology enables automated multi-colour confocal fluorescence imaging that allow detailed analysis of complex samples to be performed more easily and quickly.
- SAPO series Super Apochromatic Objectives: Converge the optic axes of red, green and blue to one focal plane; correcting the axial chromatic aberration of violet light, the original colour of samples such as red, green and blue can be presented. The resolution and brightness are improved based on large numerical aperture. Wide spectrum correction ensures flat field and increased the colour difference correction range from 400nm to 1000nm
- Software for Laser Confocal: Supports multi-channel acquisition, Z-stack imaging and large fluorescence image stitching, providing a comprehensive imaging solution.
- For image processing, the software integrates functions such as image fusion, image enhancement, 3D reconstruction and dynamic image generation, significantly improve image presentation quality. Image analysis is enhanced with advanced features like optical density and colocalization analysis.
- BCF29X Series Confocal Microscope Full Catalog
- Confocal Microscope Selection Guide
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